Pengarang
Aidil Onasis, Sugeng Juwono Mardihusodo
Deskripsi
Temephos has been applied since 1976s in Padang. Susceptibility status has been never conducted. The objective of research investigated Aedes aegypti susceptibility status to temephos in subdistrict, and spatial study of the risky area toward Dengue Haemorrhagic Fever (DHF) occurrance. The research is observational larvae density, quasi experimental to 3th and 4th larvae of Ae. aegypti collected with ovitrap and colonization for biochemically test with colour eyescore and ELISA reader λ: 450 nm. The susceptibility status was detected from esterase enzyme elevated activity (α-NA and β-NA) showing a change of colour reaction of the mosquito homogenate in microplate wells and filter paper spot tecnique. Appropriate of susceptibility from colour changes to dark blue, the susceptibility level with presented from sensitive (SS), tolerance (RS) to resistance (RR).
The research finding indicates that larvae and esterase enzyme activity elevated with substrat α-NA, by colour eyescore SS= 30, 33%, RS= 49, 00% and RR= 20, 67% with mean rate for Padang 2, 0 and it is appropriate to tolerance level. The result with ELISA reader indicates AV< 0,700= 77, 67%, AV 0,700-0,900= 18, 66% and AV> 0,900= 3, 67%. The statistic analysis of Oneway ANOVA significance 5% indicates that Ae. aegypti declining status of temephos, and multicomparison of PostHoc Test indicates difference real meaning susceptibility status between subdistrict Andalas, Jati, and Teluk Bayur from other subdistrict. The spatial study explain of the risky area toward DHF occurence based on Container Index (CI), obtained by result 35, 72% low risk and 32, 14% high risk, and CI …

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